Journal: bioRxiv

Article Title: Single cell transcriptomics of human epidermis reveals basal stem cell transition states

doi: 10.1101/784579

Figure Lengend Snippet: A) Heatmap showing top 300 differentially expressed genes per cluster. Dotted lines outline differentially expressed genes. Select marker genes are shown on the right. B) Violin plots of relative expression of marker genes split by cell cohorts and color-coded by cell community as in A. C) Feature plots showing expression of select basal and granular cell marker genes. D) Immunostaining of KRT14 (red), KRT10 (green), and DAPI (blue) in human neonatal skin. Scale bar 100µm. E-J) Immunostaining of differentially expressed proteins (red) from the (E) BAS-III cluster (ASS1 and COL17A1), (F) BAS-I cluster (PTTG1 and CDC20), (G) BAS-III/IV clusters (KRT19 and GJB2), (H) BAS-II cluster (RRM2 and PCLAF), (I) GRN cluster (SPINK5 and CALML5), and (J) the MEL (MLANA) and LAN (CD74) clusters in human neonatal skin. COL7A1 (green) and DAPI (blue) are co-stained. Dotted lines denote position of basement membrane where COL7A1 staining is absent. Scale bar 100µm.

Article Snippet: The following antibodies were used: chicken anti-KRT14 (1:500; BioLegend; SIG-3476), mouse anti-KRT10 (1:500; Dako; M7002), rabbit anti-KI67 (1:500; Abcam; ab15580), rabbit anti-PTTG1 (1:100; Sigma-Aldrich; HPA008890), mouse anti-ASS1 (1:100; Santa Cruz; sc-365475), rabbit anti-COL17A1 (1:100; One World Labs; ap9099c), rabbit anti-COL7A1 (1:500; abcam; ab93350), mouse anti-COL7A1 (1:100; Santa Cruz; sc-33710), rabbit anti-GJB2 (1:250; ThermoFisher; 51-2800), rabbit anti-KRT19 (1:250; Cell signaling; 13092), mouse anti-SPINK5 (1:100; Santa Cruz; sc-137109), mouse anti-CALML5 (1:100; Santa Cruz; sc-393637), mouse anti-CDC20 (1:100; Santa Cruz; sc-13162), mouse anti-RRM2 (1:100; Santa Cruz; sc-398294, sc-376973), mouse anti-PCLAF (1:100; Santa Cruz; sc-390515), mouse anti-MLANA (1:100; Santa Cruz; sc-20032), and mouse anti-CD74 (1:100; Santa Cruz; sc-6262).

Techniques: Marker, Expressing, Immunostaining, Staining

Journal: Structure (London, England : 1993)

Article Title: Influence of domain interactions on conformational mobility of the progesterone receptor detected by hydrogen/deuterium exchange mass spectrometry

doi: 10.1016/j.str.2014.04.013

Figure Lengend Snippet: Purified PR-A, PR-NTD, PR-LBD, TBPC or an equal molar mixture of PR-A:TBPC, PR-A NTD:TBPC, and PR-LBD:TBPC proteins were subjected to limited proteolysis with trypsin and samples were analyzed by Coomassie blue-stained SDS-PAGE or by immunoblotting. a) Coomassie-stained SDS-PAGE. b) Immunoblotting of limited proteolytic products derived from either full length PR-A or PR-A NTD with antibodies (1294 and 636) specific to distinct epitopes in the NTD or the C-terminal LBD (10A9), c) Immunoblotting with the C-terminal MAb (10A9) of limited proteolytic products generated from the isolated PR-LBD at different time points indicated (0 to 20 min).

Article Snippet: Mouse monoclonal antibodies (MAbs) to human PR (clone #1294 and 636), that detect distinct epitopes in the NTD ( Press et al., 2002 ), and clone 10A9 (Immunotech, Hamburg, Germany) elicited against amino acids 922–933, which form the extreme C-terminus of the human PR were used to detect the peptide products of PR.

Techniques: Purification, Staining, SDS Page, Western Blot, Derivative Assay, Generated, Isolation

Journal: Frontiers in Endocrinology

Article Title: PKC-mediated phosphorylation and activation of the MEK/ERK pathway as a mechanism of acquired trastuzumab resistance in HER2-positive breast cancer

doi: 10.3389/fendo.2022.1010092

Figure Lengend Snippet: Selected antibodies, fluorescence intensities and Log 2 FC in protein & phosphoprotein quantities in T15 and JIMT-1 relative to SKBR3.

Article Snippet: PI3K , PDK1 , Cell Signaling , 3062 , Rb pAb , 1398 , 808 , 1294 , 0.79 , 0.68.

Techniques: Fluorescence